In this video we introduce our series on how to perform flow cytometry data analysis.
It is part of a series of videos which will look at flow cytometry, its applications, and how you can use Tercen, a new cytometry analysis platform, to apply the latest statistical techniques to high dimensional data.
We talk about the changes in flow cytometry data analysis technology which have driven an explosion of data which cannot be analysed using the traditional methods.
We clarify the often-confusing terminology biologists have to deal with when dealing with statistical models and we outline some best practices for designing experiments which will yield results which lend themselves to better data analysis.
Flow cytometry works by suspending cells conjugated with fluorochromes in a carrier fluid. These are then passed under a laser in the flow cytometer to measure how the light acts within the machine and detect scattering and reflection.
This allows the shape and density of the cells to be measured by one detector. Another detector picks up on the wavelengths of the fluorochromes in order to identify the marker antibodies fixated to the surface of the cell.
Table of Contents:
00:00 - Introduction to Flow Cytometry
00:24 - Overview of Flow Cytometry Analysis Terminology
01:09 - CyTOF Overview
02:27 - More Flow Cytometry Terminology Explained
03:31 - Experimental design